• The state of the art NMR facility has Bruker’s AVANCE-III 500MHz and 400MHz FT NMR spectrometers. The spectrometers are equipped with BBFO & TXI-inverse probes, auto sampler and variable temperature unit. The NMR facility also houses Bruker’s AVANCE- III 700MHZ FT NMR spectrometer. The spectrometer is equipped with AscendTM superconducting magnet system, two cryo-probes (5mm TCI & 1.7mm TCI with ATMs) and one TXI-inverse probe with ATM. It also has a variable temperature system with BCU-II unit.



  • A Bruker microTOF QII high-resolution mass spectrometer is coupled to Waters Acquity UPLC system. The instrument is capable of analysing most type of samples including solid samples.


  • The GC-MS system from Agilent is one of the best instruments available in the category. It comprises of Agilent 7890A GC with Agilent 5975C MS with a dual detector mode (FID and MS together). The MS is a single quadrupole system with mass range up to 1040 u with synchronous SIM/Scan. The MS ionization can be switched between EI and CI modes. With the EI mode, ionization up to 240 eV is possible.


  • A brand new MALDI TOF spectrometer has been installed in the CIF mass facility. Combining true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh performance and extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications. The smartbeam laser provides unprecedented analytical and matrix flexibility in workflows from protein tissue imaging, intact proteins analysis, Glycoproteomics, biologics or oligo QC, to LC-MALDI proteomics - all fully enabled at 1-2000 Hz repetition rates. The high efficiency and sensitivity of the LID-LIFT process delivers MS/MS spectra with nominal mass resolution for peptides. Typically, full MS/MS data sets can be acquired with up to 1000 Hz laser repetition rate from low fmol levels within seconds. The software is perfectly adapted to analyze and visualize the match between the raw spectra and annotated peptide and protein structures. The offline coupling of liquid chromatography (LC) to MALDI mass spectrometry (LC-MALDI) has been shown to be a relevant technique in proteomics, comprising protein profiling and protein identification capabilities. Major advantages are the reduction of complexity and selection of precursor ions for the MS/MS data acquisition without any time constrains. The PROTEINEER fc II enables automatic liquid handling for MALDI preparation of LC separated peptide fractions. It automates the Proteomics workflow from LC-separation to mass spectrometric identification and characterization by MALDI TOF and TOF/TOF systems.

GC-QTOF And LC-Single Quadrupole

  • The other two instruments in the mass facility include GC-QTOF and LC-single quadrupole spectrometer from Agilent. The GC-QTOF is capable of providing HRMS for various kinds of samples and the LC-Single quadrupole mass spectrometer can handle various types of samples including biological molecules. It includes APCI option as well.


Single Crystal XRD

  • Single crystal X-ray Diffractometer (3-circle) having low temperature facility (Nitrogen cryogenics) for the determination of the crystal structure of compounds which are solids at room temperature.

    The second SC-XRD machine in the facility (D8 Venture) has Dual X-ray source, where two type of x-ray sources having micro focus x-ray diffraction property is present, Copper source for protein crystal and crystal with bigger unit cell while molybdenum for metal crystal and small unit cell containing crystal. Both x-ray tubes are air cooled and hence highly economical.Rotating anode present in the source provides highest intensities for line, spot and micro focus application. The detector (Photon 100) works on complementary metal–oxide–semiconductor technology to give higher sensitivity. The detector works at -150celsius by air cooling system. The goniometer – D8 Venture provide 2 theta , omega, phi and kappa rotation during data collection. The software is an advanced version of apex 2 in which cryo system camera system is in built and helps to work through one window. If required, temperature of 100 K can be maintained on the crystal during data collection. Due to this anisotropic displacement parameter of the crystal is decreased.

Powder XRD

  • PANalytical Empyrean XRD is a multi-purpose research X-ray diffractometer. It is unique in its ability to measure sample (powders and thin films) on a single instrument without compromising data quality. This instrument can be switched between application setups in a fast and cost-effective way using PreFIX concept, with no system realignment, and no compromise on the quality of diffraction data.


  • The protein X-ray diffractometer is equipped with high brilliance home source, MAR image plate and Oxford Cryo system. Crystallization incubators (RUMED) with grease applicator system are currently functioning at the ITI campus.X-ray source: Gen-RUH3RHB (High Brilliance) Rotating Anode (Copper) Generator with Osmic Maxflux Confocal Multilayer mirrors.Detector: mar345 Imaging Plate X-ray Data Collection System.Low Temperature Data collection: Oxford Cryosystems Cryostream Cooler, 700 series.


Size Exclusion Chromatography

  • An Agilent PL-GPC50 Plus Integrated SEC/GPC system with triple detectors (RI, PL-BV 400 viscometer and PL-RTLS dual angle light scattering).

Dynamic Light Scattering / Zeta Potential Measurement

  • Beckman Coulter Delso Nano C instrument for size analysis measurements and electrophoretic light scattering (ELS), which determines electrophoretic movement of charged particles under an applied electric field from the Doppler shift of scattered light, for zeta potential determination. This instrument is capable of measuring hydrodynamic radii in 1-7000 nm range and molecular weight as small as 267 Dalton in a concentration range from 10ppm to 40%* (w/v).

Thermogravimetric Analyzer

  • Thermogravimetric analysis is used for the investigation of physical properties of compounds such as sample volatility, moisture content, oxidation stability, decomposition temperature and flammability studies. It measures the changes in weight in relation to change in temperature extremely precisely and accurately. Scanning rates from 0.1 to 200°C/min Temperature range 15°C to 1000°C.

Isothermal Titration Calorimeter

  • Used for measuring biomolecular interactions, ITC simultaneously determines all binding parameters (n, K, H and S) in a single experiment. The TA Instruments Nano ITC is focused on the measurement of ligand binding and reaction kinetics, with increased baseline stability and more tolerance to environment temperature fluctuations. Nano ITC uses a differential power compensation design for maximum sensitivity and responsiveness. The effective cell volume of the gold cells is 190 L. It is capable of both batch/incremental titration in the temperature range 2 – 800C. The injection syringe has a capacity of 50 L and a stirring rate of 150 – 400 rpm.

Differential Scanning Calorimeter

  • Used for the studying phase transitions, such as melting, glass transitions, or exothermic decompositions. It measures the difference in the amount of heat required to increase the temperature of a sample and reference as a function of temperature extremely accurately. Temperature range -100 °C to 450 °C (having intra-cooler facility) Controlled heating rate 0.01 to 100 °C/min.

CHNSO Analyzer

  • Fully Automated PC controlled Elemental Analyser for Solids, Liquid and liquid volatile samples. Operating modes for measurement of CHNS, CHN CNS, S, Trace S and O-TCD and Cl separately.

Atomic Force Microscope

  • Agilent 5500 AFM/SPM is capable of Contact mode AFM, Lateral Force Mode (LFM), Resonant Mode AFM (semicontact + noncontact AFM), Phase Imaging, Force Modulation Microscopy, Scanning Kelvin Probe Microscopy (SKM), Magnetic Force Mode and Electrostatic Force Mode (MFM/ EFM), Scanning Tunneling Microscopy with low current capability having user-selectable gain range. It comes with a heating stage that can go upto 1500C.

BET Surface Area Measurement

  • Volumetric gas adsorption method with automated real time dead volume measurement. Measures surface area from 0.01m2/g and above (N2/77K), 0.0005 m2/g and above (Kr/77K). Measures pore size ranging from 0.32 - 500 nm in pore diameter. It can measure up to 1000 data points (per station), adsorption and/or desorption. It can measure BET, Langmuir, STSA, DFT, BJH etc. The chemisorptions mode enables unattended chemisorption measurement of gases such as CO & H2 which allows catalysis evaluation such as metal dispersion, active metal surface and average particle size of supported metal by volumetric method.

Superconducting Quantum Interference

  • Device Vibrating Sample Magnetometer with the sensitivity of a SQUID (Superconducting Quantum Interference Device) magnetometer. The SQUID VSM is equipped with a Pulse Tube Cooler which eliminates the need for liquid cryogens.

High Resolution (HR) Raman Microspectrometer

  • The HR Raman spectrometer installed at CIF is an Integrated Raman system that includes a confocal microscope, a large focal length (~800mm) Czerny-Turner type achromatic spectrograph with spectral resolution of 0.4 cm-1/pixel operating at the spectral range of 200–1050nm and the source of excitation is 632.8 nm, air cooled He-Ne laser. The instrument is capable of measuring the Raman signal for thin films, solid and solutions.


  • The EPR facility in CIF comprises of an X and Q-band capable instrument. Variable temperature accessories for both liquid nitrogen and liquid helium temperatures are available. The facility has ENDOR accessories as well.

Gel Permeation Chomatographer

Dynamic Light Scattering/Zeta Potential

Thermogravimetric Analyser (TGA 4000)

Isothermal Titration Calorimeter (Nano ITC)

Differential Scanning Calorimeter (DSC 4000)

Elemental Analyser, CHNSO

Atomic Force Microscope

Surface Area And Gas And Vapour Adsorption Measurement Instrument

Physical Property Measurement System

Superconducting Quantum Interference Device Magnetometer

Confocal Microscope With Two-Photon Laser Attachment

High Resolution Raman Microspectrometer

Protein XRD

Circular Dichroism


Office Staff

Ultrafast Laser System

  • The Ultrafast Laser system is capable of carrying out Transient Absorption and Fluorescence Up-conversion experiments. The Transient Absorption Laser System: Spitfire Ti:Sapphire regenerative ultrafast amplifier system delivers 120 fs pulses in the wavelength range of 780-120 nm with an average pulse/energy of 4mJ at a repetition rate of 1KHz. Further, output of the regenerative amplifier is coupled to Optical Parametric Amplifier, TOPAS to extend the wavelength tunability from 400-2000nm.The Fluorescence Up-conversion Laser System: Fluorescence Up-conversion, also known as fluorescence sum frequency generation, works on the principle of frequency mixing of fluorescence, originating from a fluorescent molecule upon excitation by an ultra-short pump pulse, with another ultra-short probe laser pulse. The fundamental for the Up-conversion is taken from MAITAI laser (Spectra Physics, ~120 fs, 80 MHz, 2.5 W at 800 nm) can be frequency doubled and is used to excite the sample and the frequency mixing is performed in a non-linear optical crystal, e.g. BBO (β-Borium Borate). The time evolution of the fluorescence is obtained by delaying the probe pulse using a mechanical delay stage. This technique of Fluorescence Up-conversion is used to study photo-physical properties of excited states.



  • The High resolution field emission scanning electron microscope (HR FESEM) is from Zeiss, model name ULTRA Plus. It comes with a GeminiÒ column that offers a theoretical resolution of 1.0 nm at 15 kV. It has an Everhart-Thornley detector for secondary electrons and an in-lens annular SE detector. In addition, an angle and energy selective EsB detector is also present. The sample stage is a 5-axes motorized eucentric stage with a specimen chamber with 330 mm diameter. There is also a sputter coater for coating non-conductive samples with gold, platinum or carbon. The Energy Dispersive X-ray spectrometer (EDAX) is manufactured by Oxford Instruments Nanoanalysis. It has a 50 mm2 Silicon Drift Detector that does not require liquid nitrogen cooling. It can detect elements from Boron onwards and has a Mn Ka resolution of 127 eV or better. Its operation is controlled by a different software (INCA).


Instruments at a glance